Shared Resources and Tools for the Community:
ZipSeq related materials:
ZipSeq Reagent Setup and QC Rev D: [updated 6/16/2023] This short guide will take you through the best practices I've found for generating the oligo-Ab conjugates described in our Nat. Methods paper and how to QC them on test cells w/ flow cytometry. It also lays out how to test hybridization of strands, titrations and photouncaging 'binariness' as well as strand sequences published and unpublished
ZipSeq Sectioning and Mounting: This short guide takes you through how to section your tissue well which is critical for the zipseq method. This document is performed on lymph nodes as an example, but works with PyMT tumors as well.
Materials from publications:
From Hu KH*, Kuhn NK*, Courau T, Tsui J, Samad B, Ha P, Kratz JP, Combes AJ, Krummel MF (2023). Transcriptional space-time mapping identifies concerted immune and stromal cell patterns and gene programs in wound healing and cancer. Cell Stem Cell
Mendeley Extended Data: DOI: 10.17632/kmmw43j2z6.3 , DOI: 10.17632/nnmb6m5p5j.2
Code on Github
Useful Links:
